Other

Part:BBa_K5315005:Design

Designed by: Shiori Kajikawa   Group: iGEM24_Grand-Tokyo   (2024-10-02)


Pgrac212 [B. subtilis]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

1. Tran DTM, Phan TTP, Doan TTN, Tran TL, Schumann W, Nguyen HD. Integrative expression vectors with P*grac* promoters for inducer-free overproduction of recombinant proteins in *Bacillus subtilis*. Biotechnol Rep (Amst). 2020 Oct 9;28:e00540. doi: 10.1016/j.btre.2020.e00540. PMID: 33163371; PMCID: PMC7599426. 2. Phan TT, Nguyen HD, Schumann W. Construction of a 5'-controllable stabilizing element (CoSE) for over-production of heterologous proteins at high levels in Bacillus subtilis. J Biotechnol. 2013 Oct 10;168(1):32-9. doi: 10.1016/j.jbiotec.2013.07.031. Epub 2013 Aug 14\. PMID: 23954327\.


References